Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide association and MaODR-based multi-locus interaction analyses reveal a susceptibility gene network for newly identified metabolic syndrome.

Genome biology·2026
Same author

Machine Learning for Establishing the Precision Prediction of Sarcopenia.

Gerontology·2026
Same author

Application of a novel approach for dementia prevalence prediction in Taiwan.

Scientific reports·2026
Same author

AI-Driven Fall Prediction across Generations: Integrating Deep Learning and Machine Learning for Young, Middle-Aged, and Older Adults.

Gerontology·2025
Same author

Correction: Forecasting outbound student mobility: A machine learning approach.

PloS one·2025
Same author

Prediction tools for assessing functional impacts of gene mutations in Clear Cell Renal Cell Carcinoma: A comparative study.

Computers in biology and medicine·2025
Same journal

Investigating Effect of Dimensional Variance on Separation of Glomerular Ultrafiltrate in a Microfluidic Environment.

IEEE transactions on nanobioscience·2026
Same journal

Green synthesis of multifunctional ZnFe<sub>2</sub>O<sub>4</sub>-MWCNT-Cellulose acetate nanocomposite for peroxidase enzyme immobilization.

IEEE transactions on nanobioscience·2026
Same journal

IoT-Enabled SnO₂-Based Humidity Sensor for Real-Time Monitoring in Neonatal Incubators.

IEEE transactions on nanobioscience·2026
Same journal

Electrokinetic and Antibiofilm Effects of Silver Nanoparticles Combined with Imipenem Against multidrug-resistant of Klebsiella pneumoniae.

IEEE transactions on nanobioscience·2026
Same journal

Bio-inspired Optofluidic Molecular Communication with Photothermally Actuated Microrobot Swarms.

IEEE transactions on nanobioscience·2026
Same journal

Nanostructured ZnO Thin Film-Based Enzymatic Biosensor for Sensitive Acetylcholine Detection in Neurological Applications.

IEEE transactions on nanobioscience·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.9K

PCR-CTPP design for enzyme-free SNP genotyping using memetic algorithm.

Li-Yeh Chuang, Yu-Huei Cheng, Cheng-Hong Yang

    IEEE Transactions on Nanobioscience
    |March 3, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Designing primers for single nucleotide polymorphism (SNP) genotyping using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is challenging. A novel memetic algorithm (MA) approach significantly improves the feasibility of designing effective PCR-CTPP primer sets.

    More Related Videos

    Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
    10:27

    Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

    Published on: November 10, 2015

    12.2K
    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
    07:24

    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

    Published on: February 10, 2023

    2.1K

    Related Experiment Videos

    Last Updated: Apr 16, 2026

    Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
    05:53

    Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

    Published on: June 21, 2018

    10.9K
    Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
    10:27

    Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

    Published on: November 10, 2015

    12.2K
    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
    07:24

    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

    Published on: February 10, 2023

    2.1K

    Area of Science:

    • Bioinformatics
    • Computational Biology
    • Molecular Biology

    Background:

    • Single nucleotide polymorphisms (SNPs) are crucial genetic markers.
    • Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is an efficient SNP genotyping method.
    • Designing feasible PCR-CTPP primers computationally presents significant challenges due to stringent melting temperature and primer constraint requirements.

    Purpose of the Study:

    • To develop a computational method for designing feasible primer sets for PCR-CTPP.
    • To address the challenges in primer design, including melting temperature uniformity and constraint adherence.
    • To improve the efficiency and success rate of SNP genotyping using PCR-CTPP.

    Main Methods:

    • A memetic algorithm (MA)-based method was developed for CTPP primer set design.
    • The algorithm was tested in silico on 288 SNPs from the SLC6A4 gene, excluding DIPs and MNPs.
    • Performance was compared against a genetic algorithm (GA)-based method.

    Main Results:

    • The MA-based method generated a higher number of feasible CTPP primer sets compared to the GA-based method.
    • The MA-CTPP method effectively evaluates critical melting temperatures and various primer constraints.
    • Successful in silico validation was performed on 288 target SNPs.

    Conclusions:

    • The proposed MA-based method offers a more effective solution for designing feasible CTPP primers.
    • This computational tool can assist researchers in molecular biology and biomedical fields with SNP genotyping.
    • The MA-CTPP algorithm is available via a user-friendly Java interface.