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Immunoreactive properties of anti-thyroglobulin autoantibodies isolated by affinity chromatography from human
Clinical and Experimental Immunology
|February 1, 1978
Abstract:
A Sepharose-coupled 19S human thyroglobulin has been used as an immunoadsorbent to isolate anti-thyroglobulin autoantibodies and to evaluate the antigen-antibody interactions. With the system proposed a high yield of active antibody molecules was obtained. It is possible to evaluate both the soluble and precipitating 'immunological interactions', thus avoiding the use of the double antibody technique.