Related Experiment Video
Updated: Apr 16, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
[Inheritable changes in miRNAs expression in HeLa cells after X-ray and mitomycin C treatment]
Abstract:
We identified 40 miRNAs with inherited aberrant expression by multiple parallel sequencing of human HeLa cells irradiated with X rays and mitomycin C. Twenty-two miRNAs were repressed and 15 miRNAs were induced after radiation and mytomycin C treatment. The expression of three miRNAs (miR-10b-5p, miR-148a-3p, and miR-340-5p) decreased after X-ray exposure and increased after mitomycin C treatment. The spectrum of aberrantly expressed miRNAs after X-ray and mitomycin C treatment is different, except for three miRNAs (mir-100-5p, miR-99b-5p, miR-501-3p), which showed the inherited decreased expression after both mutagens. It has been ascertained that for five miRNAs (miR-21-3p, miR-182-5p, miR-19b-3p, miR-30a-3p, and miR-30e-3p) with increased inherited expression, the targets are well-described tumor suppressor genes. For 9 miRNAs (miR-99b-5p, miR-148a-3p, miR-365a-3p, miR-193a-3p, miR-100-5p, miR-99a-5p, miR-29b-3p, miR-340-5p, and miR-23b-3p) with reduced inherited expression, the targets are oncogenes. The obtained results provide further support of the idea that induced epigenetic changes in the genome should be considered when assessing the long-term genetic effects of ionizing radiation and chemical compounds.
Insights
Ionizing radiation and mitomycin C alter microRNA (miRNA) expression in HeLa cells, impacting tumor suppressor genes and oncogenes. These findings highlight the role of epigenetic changes in long-term radiation effects.
Area of Science:
- Genomics
- Molecular Biology
- Epigenetics
Context:
- MicroRNAs (miRNAs) play crucial roles in gene regulation.
- Environmental mutagens like X-rays and mitomycin C can induce genomic instability.
- Understanding miRNA expression changes is vital for assessing long-term cellular damage.
Purpose:
- To identify microRNAs (miRNAs) with aberrant inherited expression in HeLa cells following X-ray and mitomycin C treatment.
- To investigate the relationship between altered miRNA expression and their gene targets, including tumor suppressor genes and oncogenes.
Summary:
- Multiple parallel sequencing identified 40 miRNAs with aberrant expression in HeLa cells after X-ray or mitomycin C exposure.
- Twenty-two miRNAs were repressed and 15 were induced; three showed differential expression patterns between the two mutagens.
- Five upregulated miRNAs targeted tumor suppressor genes, while nine downregulated miRNAs targeted oncogenes, suggesting a link between miRNA dysregulation and cancer-related pathways.
Impact:
- The study provides evidence that induced epigenetic changes, specifically miRNA alterations, are significant in the long-term genetic effects of radiation and chemical mutagens.
- Findings contribute to understanding the molecular mechanisms underlying cellular responses to genotoxic stress.
- Highlights the potential of miRNAs as biomarkers for radiation exposure and associated health risks.
More Related Videos
10:47Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
Published on: May 5, 2023
06:00Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
Published on: May 14, 2016
Related Concept Videos
01:22MicroRNAs
01:22MicroRNAs