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Updated: Apr 16, 2026

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Published on: May 13, 2017
Electron microscopic observation of selective excitation of conformational change of a single organic molecule
Ricardo M Gorgoll1, Emrah Yücelen2, Akihito Kumamoto3
1†Department of Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Abstract:
Atomic resolution transmission electron microscopic observations at different electron acceleration voltages enabled us to observe visually the energy relaxation process of one conformer into another via rotation of various parts of the molecule. Cross-correlation analysis of sequential transmission electron microscopy (TEM) images or of the difference between experimental and simulated TEM images has been utilized for investigation of the conformational mobility and for structure identification of conformers.
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