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On-chip graphene oxide aptasensor for multiple protein detection.

Yuko Ueno1, Kazuaki Furukawa1, Kota Matsuo2

  • 1NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan.

Analytica Chimica Acta
|March 4, 2015
PubMed
Summary
This summary is machine-generated.

This study demonstrates a versatile graphene oxide aptasensor for detecting multiple proteins like thrombin and PSA. By simply changing aptamers, the sensor enables adaptable, on-chip multicomponent analysis for diverse targets.

Keywords:
AptamerBiosensorFluorescenceGraphene oxideMicrochannelOligonucleotide

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Area of Science:

  • Biosensing
  • Nanomaterials
  • Biotechnology

Background:

  • Graphene oxide (GO) is a promising material for biosensor development due to its unique properties.
  • Aptasensors offer high specificity and sensitivity for biomolecule detection.
  • Developing versatile platforms for simultaneous detection of multiple analytes is crucial for diagnostics.

Purpose of the Study:

  • To confirm the versatility of an on-chip graphene oxide (GO) aptasensor for detecting multiple proteins.
  • To investigate the feasibility of using both DNA and RNA aptamers on a GO surface.
  • To demonstrate the potential for multicomponent analysis using a single aptasensor platform.

Main Methods:

  • Fabrication of an on-chip GO aptasensor.
  • Immobilization of specific DNA and RNA aptamers for thrombin (TB), prostate specific antigen (PSA), and hemagglutinin (HA).
  • Testing sensor performance using dual, triple, and quintuple microchannel configurations and a 2x3 linear-array GO aptasensor for selectivity and multiplexing.

Main Results:

  • The GO aptasensor successfully detected TB, PSA, and HA by simply changing the aptamers, confirming sensor versatility.
  • Both DNA and RNA aptamers immobilized on the GO surface demonstrated sufficient activity for aptasensing.
  • Molecular selectivity and concentration dependence were confirmed, and multiplexed detection of TB and PSA on a single chip was achieved.

Conclusions:

  • The developed on-chip GO aptasensor exhibits high versatility, allowing for the detection of various protein targets.
  • The platform supports both DNA and RNA aptamers, broadening its applicability.
  • This work paves the way for developing advanced multicomponent analysis systems for diverse diagnostic applications.