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Related Experiment Video

Updated: Apr 16, 2026

Polysome Purification from Soybean Symbiotic Nodules
07:02

Polysome Purification from Soybean Symbiotic Nodules

Published on: July 1, 2022

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Purification of polysomes.

Maria C Rivera1, Bruce Maguire2, James A Lake3

  • 1Department of Biology and Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia 23284;

Cold Spring Harbor Protocols
|March 4, 2015
PubMed
Summary
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This study details a sucrose gradient sedimentation protocol to isolate prokaryotic and plant polysomes (translating ribosomes). This method effectively separates polysomes from individual ribosome monomers for in vitro protein synthesis studies.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Polysomes, or translating ribosomes, are crucial for in vitro studies on protein synthesis regulation.
  • Existing methods for polysome isolation can be improved for better separation and quantification.

Purpose of the Study:

  • To present a refined protocol for isolating prokaryotic and plant polysomes using sucrose gradient sedimentation.
  • To enable the separation of polysomes from ribosome monomers and subunits.

Main Methods:

  • Sucrose gradient sedimentation was employed for polysome isolation.
  • The protocol was applied to both prokaryotic and plant biological samples.
  • Fractionation of sucrose gradients allowed for detailed analysis.

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Last Updated: Apr 16, 2026

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Published on: July 1, 2022

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Main Results:

  • The protocol successfully isolated polysomes, separating them from run-off ribosome monomers.
  • Fractionation enabled the isolation and quantification of polysomes, ribosome monomers, and ribosomal subunits.
  • The method is applicable to both prokaryotic and plant systems.

Conclusions:

  • This sucrose gradient protocol provides an effective means for isolating and characterizing polysomes.
  • The ability to separate and quantify different ribosome populations is valuable for studying protein synthesis.
  • This method enhances the toolkit for in vitro research on translational regulation.