Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

c-erbB-2 amplification in node-negative human breast cancer.

J S Ro1, A el-Naggar, J Y Ro

  • 1Department of Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030.

Cancer Research
|December 15, 1989
PubMed
Summary

c-erbB-2 gene amplification occurred in 20% of node-negative breast cancers. Amplification was linked to aneuploidy and poor nuclear grade, suggesting it may predict recurrence risk.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pooled ctDNA analysis of MONALEESA phase III advanced breast cancer trials.

Annals of oncology : official journal of the European Society for Medical Oncology·2023
Same author

Long-term histopathological changes of the nasal mucosa after total laryngectomy: a prospective cohort study.

Rhinology·2022
Same author

Third window approach assisted middle meatal antrostomy: prospective cohort study of the two ports technique in management of hard to reach maxillary sinus pathology.

The Journal of laryngology and otology·2020
Same author

Blood Hematological and Biochemical Constituents, Antioxidant Enzymes, Immunity and Lymphoid Organs of Broiler Chicks Supplemented with Propolis, Bee Pollen and Mannan Oligosaccharides Continuously or Intermittently.

Poultry science·2017
Same author

[Not Available].

Progres en urologie : journal de l'Association francaise d'urologie et de la Societe francaise d'urologie·2015
Same author

Magnetic resonance imaging features of hip disorders in an Egyptian pediatric population.

Reumatismo·2015

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The c-erbB-2 gene, also known as HER2, plays a role in cell growth and is implicated in various cancers.
  • Understanding gene amplification patterns is crucial for predicting breast cancer prognosis.

Purpose of the Study:

  • To investigate the frequency of c-erbB-2 gene amplification in node-negative breast cancer.
  • To analyze the correlation between c-erbB-2 amplification and established prognostic factors.
  • To evaluate the potential of c-erbB-2 amplification as a prognostic marker.

Main Methods:

  • Southern blot and DNA dot blot techniques were used to analyze c-erbB-2 gene amplification.
  • Tumor samples from 66 node-negative breast cancer patients were examined.
  • Analysis included correlation with nuclear grade, ploidy, estrogen receptor status, tumor size, histology, and patient age.

Related Experiment Videos

Main Results:

  • c-erbB-2 gene amplification was detected in 20% (13/66) of tumors.
  • Amplification was more frequent in aneuploid tumors (27%) and diploid tumors (14%).
  • c-erbB-2 amplification was observed in 24% of tumors with nuclear Grade 1 or 2, but not in Grade 3.
  • Patients with c-erbB-2 amplification showed higher rates of recurrence (46%) and death from metastatic disease (38%) compared to those without amplification.

Conclusions:

  • c-erbB-2 gene amplification is more prevalent in aneuploid and poorly differentiated node-negative breast cancers.
  • c-erbB-2 amplification may serve as a significant prognostic indicator for recurrence and survival in node-negative breast cancer patients.