Regulation of chicken alpha and beta actin genes and their hybrids inserted into myogenic mouse cells

We have investigated the regulation of intact non-muscle (beta) and muscle-specific (skeletal alpha) chicken actin genes and of hybrids of these two genes (alpha 5'-beta 3' and beta 5'-alpha 3') transferred into the mouse myogenic non-fusing cell line BC3H1. BC3H1 cells express members of the actin multigene family in a differentiation-dependent manner. When proliferating, the cells accumulate large amounts of non-muscle actin mRNA; when the cells are induced to differentiate, the amount of non-muscle actin mRNA decreases and the amount of muscle-specific actin mRNA increases. The transferred beta-actin gene is efficiently expressed in undifferentiated cells and appropriately down-regulated upon differentiation. In contrast, the transferred alpha-actin gene is inefficiently expressed and not consistently up-regulated. Results with the intact and hybrid genes, taken together, are consistent with the hypothesis that both 5' and 3' halves of these genes contain sequences important in regulating the efficiency and/or developmental timing of their expression in BC3H1 cells. By nuclear run-on experiments we found no evidence for gene-specific changes in the rate of transcription of the transferred actin genes during myogenesis. We conclude that the differentiation-dependent changes in expression of the intact beta-actin gene in BC3H1 cells must be regulated at the post-transcriptional level.