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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
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A Nonlinear Size-Dependent Equivalent Circuit Model for Single-Cell Electroporation on Microfluidic Chips.

Hooman Shagoshtasbi1, Peigang Deng2, Yi-Kuen Lee3

  • 1Department of Mechanical and Aerospace Engineering, HKUST, Hong Kong, China.

Journal of Laboratory Automation
|March 5, 2015
PubMed
Summary

A new model simulates electroporation (EP) dynamics in single cells on microfluidic chips. This advanced model accurately predicts cell membrane changes during EP, aiding in understanding cell permeabilization and electric breakdown.

Keywords:
electroporeequivalent circuit modelmembranemicrofluidicssingle-cell electroporation

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microfluidics

Background:

  • Electroporation (EP) utilizes electric fields to induce temporary pores in cell membranes.
  • Understanding cell electromechanical behavior during EP is crucial for applications like drug delivery and gene therapy.
  • Existing models may not fully capture the dynamic, size-dependent nature of cellular responses to EP.

Purpose of the Study:

  • To develop and validate a nonlinear, size-dependent equivalent circuit model for single-cell electroporation.
  • To investigate the dynamic electromechanical behavior of cells within a microfluidic chip during EP.
  • To accurately predict cell membrane permeabilization stages and electric breakdown.

Main Methods:

  • A nonlinear size-dependent equivalent circuit model was developed, incorporating cell components, poration media, and microfluidic chip elements.
  • A single-cell microfluidic EP chip with 3D microelectrode arrays was designed and fabricated for experimental validation.
  • Model predictions of electrical current were compared against experimental single-cell EP data.

Main Results:

  • The model demonstrated good agreement with experimental electrical current responses (average error of 6%).
  • The model accurately predicted transmembrane voltage, pore diameter, and pore density across four distinct permeabilization stages.
  • Current-voltage characteristics and electric breakdown voltages for different cell lines were precisely predicted.

Conclusions:

  • The proposed size-dependent model effectively captures the complex electromechanical behavior of single cells during electroporation.
  • This model provides a valuable tool for analyzing cell permeabilization dynamics and predicting electric breakdown thresholds.
  • The findings advance the understanding and application of electroporation in microfluidic systems.