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Updated: Sep 9, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors
Published on: July 31, 2014
The N-terminal cysteine of human asparagine synthetase is essential for glutamine-dependent activity
1Department of Chemistry, University of Nebraska, Lincoln 68588.
Abstract:
Site-specific mutagenesis was used to replace the N-terminal cysteine in human asparagine synthetase by an alanine. The mutant enzyme was expressed in the yeast Saccharomyces cerevisiae, and the asparagine synthetase activity was analyzed in vitro. The mutation resulted in the loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected. These results confirm the existence of a glutamine amidotransfer domain with an N-terminal cysteine essential for the glutamine-dependent asparagine synthetase activity.
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