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Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
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Non-derivatized glycan analysis by reverse phase liquid chromatography and ion mobility-mass spectrometry
Nichole M Lareau1, Jody C May, John A McLean
1Department of Chemistry, Center for Innovative Technology, Institute of Chemical Biology, and Institute for Integrative Biosystems Research and Education, Vanderbilt University, 7330 Stevenson Center, Nashville, TN 37235, USA. john.a.mclean@vanderbilt.edu.
The Analyst
|March 5, 2015
Abstract:
A simple method for the analysis of non-derivatized glycans using a reverse phase column on a liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) instrument. The methodology supports both glycomic and proteomic work flows without the necessity of switching columns.

