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Updated: Apr 16, 2026
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
A subset of circulating microRNAs is expressed differently in patients with myocardial infarction
Jianqiu Liang1, Shuchang Bai2, Liang Su1
1Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Abstract:
The objective of the present study was to analyze the differences in the plasma microRNA (miRNA) expression profiles between patients with myocardial infarction (MI) (with or without heart failure) and individuals in a normal control group using an miRNA array. Specific miRNAs were selected to explore novel circulating markers for MI and heart failure. A total of 15 patients with heart failure and 10 patients without heart failure following acute MI (AMI) were recruited as the AMI with heart failure (AMHF) and with no heart failure (AMNHF) groups, respectively. In addition, 10 patients with an older (≥ 1 year) MI with heart failure were selected as the old MI and heart failure (OMHF) group. Finally, 10 patients with normal coronary angiograms were recruited as the control (N) group. The plasma of peripheral venous blood was collected for miRNA array detection. In the AMHF group, the expression of 17 miRNAs was upregulated and the expression of 21 miRNAs was downregulated by >1.5-fold compared with that in the AMNHF group. Compared with the N group, the expression of miRNAs in the AMNHF group was upregulated in 38 and downregulated in 48 cases by >1.5-fold. Compared with the OMHF group, 13 miRNAs were upregulated and 43 were downregulated by >1.5-fold in the AMHF group. Significant differences in the miRNA expression profiles were observed between patients with different stages of heart failure following MI and individuals in the normal control group. These differences were determined using miRNA array analysis methods based on the peripheral blood plasma. Thus, the specific miRNAs identified in this study may be novel circulating markers for MI and heart failure.
Insights
This study identified distinct plasma microRNA (miRNA) profiles in patients with myocardial infarction (MI) and heart failure, revealing potential new circulating biomarkers for these conditions.
Area of Science:
- Biochemistry
- Cardiology
- Molecular Biology
Background:
- Myocardial infarction (MI) and subsequent heart failure represent significant global health challenges.
- Early and accurate diagnosis is crucial for effective patient management and improved outcomes.
- Circulating microRNAs (miRNAs) are emerging as promising biomarkers for various cardiovascular diseases.
Purpose of the Study:
- To investigate plasma miRNA expression profiles in patients with acute myocardial infarction (AMI) with and without heart failure compared to controls.
- To identify novel circulating miRNA biomarkers for myocardial infarction and heart failure.
- To analyze differences in miRNA expression between acute and old myocardial infarction with heart failure.
Main Methods:
- Plasma samples were collected from four groups: AMI with heart failure (AMHF), AMI without heart failure (AMNHF), old MI with heart failure (OMHF), and normal controls (N).
- MicroRNA (miRNA) expression profiling was performed using an miRNA array.
- Statistical analysis was employed to identify differentially expressed miRNAs (>1.5-fold change).
Main Results:
- Significant differences in plasma miRNA expression were observed between all patient groups and the control group.
- Compared to AMNHF, 17 miRNAs were upregulated and 21 downregulated in AMHF.
- Compared to controls, 38 miRNAs were upregulated and 48 downregulated in AMNHF; 13 miRNAs were upregulated and 43 downregulated in AMHF compared to OMHF.
Conclusions:
- Plasma miRNA expression profiles differ significantly in patients with myocardial infarction and varying stages of heart failure.
- Specific miRNAs identified in this study hold potential as novel circulating biomarkers for the diagnosis of MI and heart failure.
- miRNA array analysis of peripheral blood plasma is a viable method for discovering these biomarkers.
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