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Digital Microfluidics for Automated Proteomic Processing
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Modular microfluidics for point-of-care protein purifications.

L J Millet1, J D Lucheon, R F Standaert

  • 1Biological and Nanoscale Systems Group, Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS 6445, Oak Ridge, TN 37831-6445, USA. milletlj@ornl.gov.

Lab on a Chip
|March 6, 2015
PubMed
Summary
This summary is machine-generated.

Miniaturized microfluidic chromatography modules enable portable biochemical separations. These on-chip modules offer comparable performance to traditional methods for protein purification and diagnostics.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Microfluidics and Lab-on-a-Chip Technology
  • Biotechnology and Bioprocessing

Background:

  • Biochemical separations are crucial for diagnostic assays and biologic purification.
  • On-chip miniaturization and modularization promise customized, portable devices for healthcare and biomanufacturing.

Purpose of the Study:

  • To design and fabricate miniature chromatography modules (ion exchange, size exclusion, affinity) for on-chip protein purification.
  • To demonstrate the integration of these modules using embedded 3-D microfluidic interconnects for reconfigurable fluidic systems.
  • To showcase the utility of this modular approach for protein enrichment from complex biological samples.

Main Methods:

  • Fabrication of microfluidic modules for ion exchange, size exclusion, and affinity chromatography.
  • Development of embedded 3-D microfluidic interconnects for module integration and reconfiguration.
  • Demonstration of a platform for enhanced green fluorescent protein (eGFP) enrichment from Escherichia coli lysate.

Main Results:

  • Miniature chromatography modules achieved performance comparable to conventional separation techniques.
  • Embedded interconnects enabled flexible arrangement and reconfiguration of microfluidic modules.
  • The modular platform successfully enriched eGFP from bacterial lysate using integrated affinity and size-exclusion chromatography.

Conclusions:

  • Microfluidic chromatography modules are viable for miniaturized and modular biochemical separations.
  • This approach facilitates the development of portable devices for personalized diagnostics and point-of-use bioproduction.
  • The reconfigurable modular system offers versatility for various fluidic operations and biochemical processes.