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Updated: Apr 16, 2026

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
Gene silencing by DNA interference in fern gametophytes
Masamitsu Wada1, Hidenori Tsuboi
1Biology Department, Faculty of Science, Kyushu University, 6-10-1 Hakozaki, Higashi Ward, Fukuoka, 812-8581, Japan, wadascb@kyushu-u.org.
Abstract:
RNA interference is commonly used for posttranscriptional silencing of target gene transcripts. In fern gametophytes, however, sequence-specific gene silencing is possible by introducing double-stranded DNA fragments into gametophyte cells by particle bombardment. Silencing could be transmitted all over the gametophyte through live cells. Further, inheritance of the gene silencing to the progeny is depending on the gene used. Here we describe how to introduce the DNA fragments into the gametophyte cells and how to screen the DNA-transferred cells.
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