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Potato Virus X-Based microRNA Silencing VbMS In Potato.
Published on: May 11, 2020
MR VIGS: microRNA-based virus-induced gene silencing in plants
Weiwei Chen1, Qi Zhang, Junhua Kong
1Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, 16 Xuelin Street, Jianggan, Zhejiang, Hangzhou, China.
Abstract:
In plants, microRNA (miRNA)-based virus-induced gene silencing, dubbed MR VIGS, is a powerful technique to delineate the biological functions of genes. By targeting to a specific sequence, miRNAs can knock down expression of genes with fewer off-target effects. Here, using a modified Cabbage leaf curling virus (CaLCuV) and Tobacco rattle virus (TRV) as vectors, we describe two virus-based miRNA expression systems to perform MR VIGS for plant functional genomics assays.
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