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Mesencephalic basolateral domain specification is dependent on Sonic Hedgehog.

Jesus E Martinez-Lopez1, Juan A Moreno-Bravo1, M Pilar Madrigal1

  • 1Instituto de Neurociencias de Alicante, Universidad Miguel Hernandez, Consejo Superior de Investigaciones Científicas (UMH-CSIC) Alicante, Spain.

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|March 6, 2015
PubMed
Summary

New molecular markers like Barhl1, Nhlh1, and Six3 help define midbrain domains. Sonic Hedgehog (Shh) is crucial for specifying the basolateral domain, as its absence causes significant alterations.

Keywords:
Barhl1Nhlh1ShhSix3alar-basal boundarybasolateral domainmidbrain

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Understanding central nervous system morphogenesis relies on identifying molecular markers for axis specification.
  • The midbrain's alar and basal plates have defined domains, but the precise alar-basal boundary remains debated.
  • Barhl1, Nhlh1, and Six3 are proposed as markers for domains near the alar-basal transition.

Purpose of the Study:

  • To identify and characterize molecular markers defining domains in the developing midbrain.
  • To investigate the role of these markers in specifying mesencephalic populations.
  • To elucidate the influence of Sonic Hedgehog (Shh) signaling on these markers and domain development.

Main Methods:

  • Expression pattern analysis of Barhl1, Nhlh1, and Six3 in the developing midbrain.
  • Assessment of mesencephalic population contributions.
  • Functional study involving the manipulation of Sonic Hedgehog (Shh) signaling.

Main Results:

  • Barhl1, Nhlh1, and Six3 were identified as markers for adjacent domains at the alar-basal transition.
  • Their expression patterns confirmed their roles in specifying distinct mesencephalic domains.
  • Shh signaling is essential for the proper expression of Barhl1 and Nhlh1, and influences Six3 distribution, impacting basolateral domain development.

Conclusions:

  • The alar-basal boundary is located dorsal to the basolateral domain.
  • The development of the basolateral domain is critically dependent on Sonic Hedgehog (Shh) signaling.
  • Barhl1, Nhlh1, and Six3 are valuable molecular markers for studying midbrain patterning and Shh-mediated development.