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Peroxidase-labeled primary antibody method for detection of pestivirus contamination in cell cultures
B J Potts1, M Sawyer, I C Shekarchi
1Infectious Diseases Branch, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
Journal of Virological Methods
|October 1, 1989
Abstract:
Contaminating bovine viral diarrhea (BVD) virus in cell cultures and in fetal bovine serum (FBS) is a well recognized problem. This study describes a direct peroxidase (DP) labeled primary antibody method for detection of pestivirus antigens in cell culture that is simple and reliable. Using this method, most bovine and porcine cell cultures, a cat lung, four mosquito and two monkey cell cultures were found contaminated with BVD virus. The rodent and human cell cultures tested were negative by this method for BVD virus.