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Next-generation Sequencing

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Updated: Apr 16, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Targeted DNA methylation analysis by next-generation sequencing.

Dustin R Masser¹, David R Stanford¹, Willard M Freeman²

¹Department of Physiology, University of Oklahoma College of Medicine.

Journal of Visualized Experiments : Jove

|March 6, 2015

Summary

This summary is machine-generated.

Bisulfite Amplicon Sequencing (BSAS) offers a rapid, quantitative method for analyzing DNA methylation in targeted genomic regions. This technique enables high-throughput epigenomic studies for researchers with basic molecular biology skills.

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Targeted DNA Methylation Analysis by Next-generation Sequencing

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Methyl-binding DNA capture Sequencing for Patient Tissues

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DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Area of Science:

Epigenetics
Molecular Biology
Genomics

Background:

DNA methylation is a key epigenetic regulator of gene expression, crucial for understanding health and disease.
Previous high-throughput methods for quantitative DNA methylation analysis were lacking.
Next-generation sequencing (NGS) technologies are increasingly applied to epigenomics.

Purpose of the Study:

To develop a rapid, quantitative, and accessible method for analyzing DNA methylation in targeted genomic regions.
To enable high-throughput epigenomic studies for researchers with basic molecular biology skills.

Main Methods:

Bisulfite Amplicon Sequencing (BSAS) combines bisulfite conversion with targeted amplification.
Utilizes transposome-mediated library construction and benchtop NGS.
Analyzes up to 10 kb of targeted regions in up to 96 samples simultaneously.

Main Results:

BSAS provides absolute quantitation of cytosine methylation with base specificity.
The method is rapid, efficient, and applicable to any DNA source.
Suitable for hypothesis testing and confirmation of genome-wide methylation findings.

Conclusions:

BSAS is a valuable tool for epigenomic research, particularly for hypothesis-driven studies on specific genomic regions.
The method democratizes quantitative methylation analysis, making it accessible to a wider range of research groups.
BSAS facilitates accurate assessment of DNA methylation patterns in various biological contexts.