Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Renal Clearance

Renal Clearance

The glomerular filtration rate (GFR) is a critical marker of kidney function, reflecting the efficiency of filtration by the glomeruli. Renal clearance of specific substances, such as inulin or creatinine, is commonly used to measure GFR.
Renal clearance refers to the volume of plasma cleared of a specific substance, such as creatinine, per unit of time. To measure clearance, urine samples are collected over a 24-hour period during each bladder voiding, followed by a single blood sample at the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Endocrine Characteristics in Bardet Biedl Syndrome from a Large Single-Centre Paediatric Multidisciplinary Clinic.

The Journal of clinical endocrinology and metabolism·2026

Same author

Hyperphagia in Bardet-Biedl syndrome: Pathophysiology, burden, and management.

Obesity reviews : an official journal of the International Association for the Study of Obesity·2025

Same author

Cell Catcher: A New Method to Extract and Preserve Live Renal Cells from Urine.

Kidney360·2024

Same author

De-Suppression of Mesenchymal Cell Identities and Variable Phenotypic Outcomes Associated with Knockout of <i>Bbs1</i>.

Cells·2023

Same author

Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice Using Endogenous Streptavidin/FLAG Tags.

Methods in molecular biology (Clifton, N.J.)·2023

Same author

IFT74 variants cause skeletal ciliopathy and motile cilia defects in mice and humans.

PLoS genetics·2023

Search research articles

Related Experiment Video

Updated: Apr 16, 2026

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

Published on: February 20, 2015

Evaluation of zebrafish kidney function using a fluorescent clearance assay.

Sonia Christou-Savina¹, Philip L Beales¹, Daniel P S Osborn²

¹Genetics and Genomic Medicine, Institute of Child Health, University College London.

Journal of Visualized Experiments : Jove

|March 6, 2015

Summary

Researchers developed a novel fluorescent assay to assess zebrafish kidney function. This method allows quantitative monitoring of dye clearance, overcoming limitations of traditional assays for studying renal development and disease in this model organism.

More Related Videos

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Published on: August 9, 2014

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Published on: May 15, 2021

Related Experiment Videos

Last Updated: Apr 16, 2026

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay

Published on: February 20, 2015

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney

Published on: August 9, 2014

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Published on: May 15, 2021

Area of Science:

Developmental biology
Nephrology
Comparative physiology

Background:

The zebrafish embryo is a valuable model for studying organogenesis and human kidney diseases due to conserved developmental pathways.
Despite differences in nephron number, zebrafish kidney structure and function closely resemble human kidneys.
Studying kidney development and disease in zebrafish is hindered by challenges in assessing renal function.

Purpose of the Study:

To develop and validate a novel, non-invasive assay for quantitatively measuring zebrafish kidney function.
To overcome the limitations of traditional human kidney function assays in the zebrafish model.
To facilitate genetic studies of kidney development and disease in zebrafish.

Main Methods:

Utilized the optical transparency of zebrafish embryos.
Developed a fluorescent assay to monitor dye clearance from the vasculature through the kidney.
Quantitatively measured dye excretion over time to assess renal function.

Main Results:

Successfully demonstrated a quantitative readout of renal function using a fluorescent dye clearance assay.
The assay overcomes the limitations of traditional methods for assessing kidney function in small aquatic models.
Provides a new tool for evaluating kidney function after genetic manipulation in zebrafish.

Conclusions:

The developed fluorescent assay offers a robust and quantitative method for assessing zebrafish kidney function.
This assay will significantly advance research into kidney development, disease modeling, and therapeutic strategies using zebrafish.
Enables more effective translation of zebrafish kidney research to human health.