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Related Experiment Video

Updated: Apr 16, 2026

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Human vascular model with defined stimulation medium - a characterization study.

Outi Huttala¹, Hanna Vuorenpää¹, Tarja Toimela¹

¹FICAM, Finnish Centre for Alternative Methods, School of Medicine, University of Tampere, Tampere, Finland.

|March 6, 2015

Summary

This summary is machine-generated.

Researchers developed a serum-free medium for a human cell-based vascular model. This advanced model mimics mature blood vessels, reducing animal testing for drug development and tissue engineering.

Keywords:

angiogenesis coculture techniques mesenchymal stromal cells serum-free media

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Last Updated: Apr 16, 2026

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Published on: April 4, 2018

Area of Science:

Biomedical Engineering
Cell Biology
Regenerative Medicine

Background:

Vascular formation is crucial for development and physiology.
Current animal-based models show species variation, limiting human relevance.
Existing human cell models lack detailed characterization.

Purpose of the Study:

To develop a well-characterized, human-relevant in vitro vascular model.
To create a defined, serum-free vascular stimulation medium (VSM).
To reduce and replace animal testing in vascular research.

Main Methods:

Co-culture of human adipose stromal cells (hASC) and human umbilical vein endothelial cells (HUVEC).
Development and application of a novel defined serum-free vascular stimulation medium (VSM).
Characterization using cell identity, maturation, structural analysis, and gene expression.

Main Results:

The VSM induced a reproducible, vascular-like network with mature vessel morphology.
Electron microscopy confirmed a three-dimensional network structure with lumens.
Significant upregulation of key human angiogenesis-related genes was observed.

Conclusions:

The new defined medium enables a thoroughly characterized in vitro vascular model.
This model serves as a reliable test system for chemical testing.
The model is suitable for developing vascularized tissue models, reducing animal use.