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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
Published on: March 24, 2012
Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry
Gongyu Li1, Jiying Pei, Yue Yin
1Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, P. R. China. gmhuang@ustc.edu.cn.
Abstract:
Dissociation of disulfide is normally mandatory prior to disulfide peptide sequencing via electrospray ionization collision induced dissociation mass spectrometry (ESI-CID-MS). Herein, a facile method for directly sequencing intact disulfide peptides was proposed. The basic principles involved were electrolyte-enhanced corona discharge in ESI and the following oxidative cleavage reaction.
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