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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

102.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Related Experiment Video

Updated: Apr 16, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

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Nanopore-based fourth-generation DNA sequencing technology.

Yanxiao Feng1, Yuechuan Zhang2, Cuifeng Ying3

  • 1Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Genomics, Proteomics & Bioinformatics
|March 7, 2015
PubMed
Summary
This summary is machine-generated.

Fourth-generation nanopore sequencing offers rapid, low-cost whole human genome sequencing. This technology enables single-molecule studies of DNA-protein and protein-protein interactions, advancing molecular biology.

Keywords:
DNA sequencingNanoporeSingle baseSingle molecule

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Nanopore sequencing represents a significant advancement in DNA sequencing technology.
  • Current limitations in sequencing cost and speed hinder widespread genomic applications.

Purpose of the Study:

  • To review the historical development and recent breakthroughs in nanopore sequencing technology.
  • To explore the potential applications of nanopore analysis in molecular biology and genomics.

Main Methods:

  • Review of academic literature on nanopore technology, encompassing both biological and solid-state nanopores.
  • Analysis of single-molecule interaction studies enabled by nanopore techniques.

Main Results:

  • Nanopore sequencing holds the potential for rapid and cost-effective whole human genome sequencing (under $1000, potentially under $100).
  • Single-molecule analysis capabilities facilitate in-depth studies of DNA-protein and protein-protein interactions.

Conclusions:

  • Nanopore technology is revolutionizing molecular biology by enabling single-molecule scale investigations.
  • Continued advancements in nanopore technology promise broader applications in genomics and beyond.