Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Pulmonary rehabilitation interventions strategies for patients with chronic obstructive pulmonary disease complicated with anxiety and depression: a summary of best evidence].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2026
Same author

Genomic Proximity Mapping for Identification of Chromosomal Aberrations in Multiple Myeloma.

American journal of hematology·2026
Same author

Genomic mechanisms of resistance to venetoclax in multiple myeloma with t(11;14)(CCND1;IGH).

Blood·2026
Same author

Multi-omics analysis reveals a unique epigenetic signature in <i>MYD88</i> wild-type Waldenström macroglobulinemia.

Haematologica·2025
Same author

Genomic profiling reveals molecular heterogeneity in patients with Richter transformation (RT) and chronic lymphocytic leukemia (CLL).

Leukemia research·2025
Same author

CHIP amplifies the risk of lymphoid malignancies in individuals with monoclonal B-cell lymphocytosis (MBL).

Blood cancer journal·2025

Related Experiment Video

Updated: Apr 16, 2026

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

7.4K

Longitudinal analysis of 25 sequential sample-pairs using a custom multiple myeloma mutation sequencing panel

K M Kortüm1, C Langer, J Monge

  • 1Division of Hematology - Oncology, Mayo Clinic, Scottsdale, AZ, USA.

Annals of Hematology
|March 7, 2015
PubMed
Summary

We developed the first multiple myeloma (MM)-specific gene panel for targeted sequencing. This panel tracks genetic mutations and clonal evolution, aiding personalized treatment strategies for MM patients.

More Related Videos

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

25.2K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.4K

Related Experiment Videos

Last Updated: Apr 16, 2026

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

7.4K
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

25.2K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.4K

Area of Science:

  • Genomics
  • Oncology
  • Molecular Biology

Background:

  • Next-generation sequencing (NGS) advances enable rapid clinical results for personalized cancer treatment.
  • No established gene panel exists for multiple myeloma (MM)-specific genomic profiling.

Purpose of the Study:

  • To design and validate a novel 47-gene targeted sequencing panel for multiple myeloma.
  • To analyze tumor/germline DNA in MM patients longitudinally to track clonal evolution.

Main Methods:

  • Designed a 47-gene panel (39 common MM mutations, 8 targeted pathway genes).
  • Performed targeted sequencing on tumor/germline DNA from 25 MM patients with pre- and post-treatment samples.

Main Results:

  • KRAS (36%), NRAS (20%), TP53 (16%), DIS3 (16%), FAM46C (12%), and SP140 (12%) were most frequently mutated.
  • Tracked clonal evolution, noting mutation changes in FAM46C, FAT1, KRAS, NRAS, SPEN, PRDM1, NEB, and TP53.
  • Identified XBP1 mutations linked to bortezomib resistance.

Conclusions:

  • The developed MM-specific gene panel enables individual tumor characterization.
  • This panel facilitates longitudinal tracking of clonal evolution in multiple myeloma.
  • Findings support the use of targeted sequencing for personalized MM treatment and monitoring.