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Related Experiment Video

Updated: Jan 9, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

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[Advanced technologies for the human gut microbiome analysis].

Masahira Hattori1

  • 1Center for Omics and Bioinformatics, Graduate School of Frontier Sciences, The University of Tokyo.

Nihon Rinsho Men'Eki Gakkai Kaishi = Japanese Journal of Clinical Immunology
|March 7, 2015
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) makes human gut microbiome analysis practical, revealing its influence on host health and disease. This study details NGS methods for characterizing gut microbial communities.

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Last Updated: Jan 9, 2026

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Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Context:

  • The human gut microbiome, a complex community of over 100 trillion microbial cells, plays a crucial role in host physiology.
  • Advances in next-generation sequencing (NGS) have revolutionized the ability to analyze the gut microbiome's genetic and species composition.
  • Previous studies highlight the microbiome's influence on health and disease, often showing aberrant structures in diseased states.

Purpose:

  • To present a standardized NGS-based methodology for human gut microbiome analysis.
  • To enable high-throughput and accurate characterization of microbial genes, functions, and species.
  • To facilitate a deeper understanding of the gut microbiome's ecological and biological features.

Summary:

  • NGS technologies provide practical and high-throughput methods for analyzing the human gut microbiome.
  • These approaches allow for comprehensive characterization of microbial genes, functions, and species composition.
  • The methodology addresses the need for accurate evaluation of microbiome data, considering biological and ecological viewpoints.

Impact:

  • Enables detailed assessment of gut microbiome structure, including the impact of experimental protocols (e.g., sample handling, DNA preparation, sequencing platforms).
  • Facilitates research into the gut microbiome's profound influence on various host physiologies and diseases.
  • Supports the identification of aberrant gut microbiome structures associated with disease states compared to healthy controls.