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A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and
Robert J Meier1, Johann M B Simbürger, Tero Soukka
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.
Chemical Communications (Cambridge, England)
|March 10, 2015
Abstract:
A luminescent probe for determination of pH was designed based on a Förster resonance energy transfer (FRET) system, combining a europium chelate as the donor and carboxynaphtho-fluorescein as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.
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