Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

17.2K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
17.2K
The Replisome03:01

The Replisome

39.9K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
39.9K
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

50.8K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
50.8K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.3K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.3K
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

4.8K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
4.8K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

15.3K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
15.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Concurrent Calcium Pyrophosphate Deposition Disease and Gout of the Cervical Spine Presenting With Subacute Neck Pain and Radiculopathy.

The Journal of rheumatology·2026
Same author

Comparative analysis of hyaluronic acid fillers: Juvéderm Volux versus MaiLi Extreme in microstructural, rheological and mechanical properties.

JPRAS open·2026
Same author

Prognostic value and potential upstream regulator of Schwann cells in esophageal squamous cell carcinoma.

Translational cancer research·2026
Same author

A Hand-Held Digital Printer for On-Site Fabrication of Micro/Nano Composite Dressings.

ACS applied materials & interfaces·2026
Same author

Nrf2/HO-1 Dysregulation is Involved in the Microplastic-Induced Gut-Liver Axis Damage Mechanism and the Protective Effect of Astaxanthin.

Journal of agricultural and food chemistry·2026
Same author

Thoracoscopic resection of the second rib via a periareolar approach: a case report.

Journal of cardiothoracic surgery·2026

Related Experiment Video

Updated: Apr 16, 2026

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
09:47

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Published on: March 1, 2012

12.8K

Host ESCRT proteins are required for bromovirus RNA replication compartment assembly and function.

Arturo Diaz1, Jiantao Zhang2, Abigail Ollwerther1

  • 1Institute for Molecular Virology, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.

Plos Pathogens
|March 10, 2015
PubMed
Summary

Brome mosaic virus (BMV) RNA replication requires the host endosomal sorting complexes required for transport (ESCRT) machinery for forming viral replication compartments. ESCRT pathway disruption inhibits BMV replication and spherule formation, highlighting its role in viral RNA replication.

More Related Videos

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

22.2K
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

4.2K

Related Experiment Videos

Last Updated: Apr 16, 2026

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
09:47

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Published on: March 1, 2012

12.8K
Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

22.2K
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

4.2K

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Positive-strand RNA virus genome replication is linked to cellular membrane rearrangements.
  • Brome mosaic virus (BMV) replication occurs in ~70 nm spherules on endoplasmic reticulum (ER) membranes.
  • Viral budding processes often utilize the host ESCRT machinery.

Purpose of the Study:

  • To investigate the role of the host ESCRT pathway in BMV RNA replication and spherule formation.
  • To determine if ESCRT components are essential for BMV replication compartments.

Main Methods:

  • Deletion of ESCRT pathway components in BMV-infected cells.
  • Analysis of BMV RNA replication levels.
  • Microscopic examination of BMV spherule formation and protein localization.

Main Results:

  • Disruption of specific ESCRT genes differentially affected BMV RNA replication and spherule formation.
  • Deletion of SNF7 severely inhibited BMV RNA replication and abolished spherule formation.
  • BMV ESCRT recruitment and spherule assembly utilize distinct protein-protein interactions compared to other viral or cellular processes.

Conclusions:

  • Cellular ESCRT components are crucial for the proper formation and function of BMV RNA replication compartments.
  • The ESCRT pathway represents a potential target for broad-spectrum antiviral therapies due to its diverse interactions with viruses.