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Apelin protects against acute renal injury by inhibiting TGF-β1

Hong Chen1, Danyang Wan2, Lin Wang2

  • 1Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China, 430030; Centre for Biomedicine Research, Wuhan Institute of Biotechnology, Wuhan, China, 430074.

Biochimica Et Biophysica Acta
|March 10, 2015
PubMed

Insights

Apelin-13 peptide effectively protects against acute kidney injury caused by renal ischemia/reperfusion (I/R). This treatment reduces kidney damage, inflammation, and cell death, suggesting its therapeutic potential.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Cell Biology

Background:

  • Renal ischemia/reperfusion (I/R) injury is a primary cause of acute kidney injury (AKI) with high mortality.
  • Current therapies for AKI lack effectiveness.
  • Apelin-13, a bioactive peptide, has demonstrated protective effects in diabetic nephropathy models.

Purpose of the Study:

  • To investigate the protective effects of apelin-13 against renal I/R injury in a rat model.
  • To explore the molecular mechanisms underlying apelin-13's renoprotective actions.

Main Methods:

  • Male rats were subjected to renal I/R injury with or without apelin-13 treatment.
  • In vitro studies utilized cultured renal mesangial and tubular cells exposed to hypoxia/reperfusion (H/R).
  • Assessed tubular lesions, apoptosis, renal dysfunction, inflammatory factors, TGF-β1, histone methylation (H3K4me2), and Kmt2d expression.

Main Results:

  • Apelin-13 treatment significantly reduced tubular lesions, renal cell apoptosis, and normalized renal dysfunction post-I/R.
  • Apelin-13 inhibited the elevation of inflammatory factors, TGF-β1, and apoptosis induced by I/R.
  • Apelin-13 suppressed I/R-induced increases in histone methylation (H3K4me2) and Kmt2d in vivo and in vitro.

Conclusions:

  • Apelin-13 demonstrates significant renoprotective effects against I/R injury by mitigating inflammation and apoptosis.
  • The mechanism involves the inhibition of TGF-β1 and aberrant histone methylation.
  • Apelin-13 presents a promising therapeutic candidate for acute kidney injury.
Keywords:
ApelinHistone methylationRenal I/R injuryTGF-β1

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