Immediate human pulp response to ethanol-wet bonding technique

Area of Science:

• Dental Materials Science
• Biomaterials
• Adhesive Dentistry

Background:

• The ethanol-wet bonding technique is an alternative to traditional water-wet bonding in restorative dentistry.
• Understanding the short-term pulpal response to ethanol-wet bonding is crucial for clinical application.

Purpose of the Study:

• To evaluate the short-term (48h) in vivo response of human pulp tissue to the ethanol-wet bonding technique.
• To compare pulpal reactions between ethanol-wet and water-wet bonding methods.

Main Methods:

• Deep Class V cavities were prepared in human premolars and divided into three groups: ethanol-wet bonding, water-wet bonding, and calcium hydroxide control.
• Teeth were restored with resin composite after acid-etching and application of Single Bond 2.
• Histological analysis (H/E, Masson's trichrome) and bacterial microleakage assessment were performed 48 hours post-procedure.

Main Results:

• No significant difference in pulpal inflammatory response was observed between ethanol-wet and water-wet bonding groups.
• Slight inflammatory responses and odontoblast layer disruption were noted in all tested groups.
• No bacterial microleakage was detected in any of the experimental groups.

Conclusions:

• The ethanol-wet bonding technique does not induce greater pulpal damage than the water-wet bonding technique at 48 hours.
• Ethanol-wet bonding presents a potential for improved resin-dentine bond durability.
• This study provides insights into the in vivo human pulp response to ethanol-wet bonding with a simplified etch-and-rinse adhesive.