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Atypical human rotaviruses in the G.D.R
Acta Virologica
|August 1, 1989
Summary
Electron microscopy detected numerous rotaviruses in children with diarrhea, even when ELISA tests were negative. Atypical rotaviruses, identified by RNA migration, were also found, suggesting group C rotaviruses as a cause.
Area of Science:
- Pediatric Gastroenterology
- Virology
- Microbiology
Background:
- Diarrhea is a common illness in young children.
- Accurate identification of causative agents is crucial for effective treatment.
Purpose of the Study:
- To identify the aetiologic agents of diarrhea in children aged one to three years.
- To investigate the presence of atypical rotaviruses.
Main Methods:
- Electron microscopy (EM) was used to detect rotaviruses in stool samples.
- Enzyme-linked immunosorbent assay (ELISA) was performed for comparison.
- Electrophoretic analysis of RNA migration profiles was used to characterize rotaviruses.
Main Results:
- A large number of rotaviruses were detected by EM, despite negative ELISA results.
- Ten conventional (group A) and 21 antigenically distinct rotaviruses were identified in 141 children.
- Electropherotype patterns confirmed the presence of atypical rotaviruses, consistent with group C rotaviruses.
Conclusions:
- Electron microscopy is a sensitive method for detecting rotaviruses in pediatric diarrhea.
- Atypical rotaviruses, potentially group C, are present in children with diarrhea.
- Further investigation into atypical rotaviruses is warranted.