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A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
Published on: May 29, 2012
Aptamer-modified magnetic beads in affinity separation of proteins
Guohong Zhu1, Johanna-Gabriela Walter
1Institut für Technische Chemie, Gottfried Wilhelm Leibniz Universität Hannover, Callinstr. 5, 30167, Hannover, Germany.
Abstract:
Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
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