Immobilized magnetic beads-based multi-target affinity selection coupled with HPLC-MS for screening active compounds
Yaqi Chen1, Zhui Chen, Yi Wang
1Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, 866 Yuhangtang Rd, Hangzhou, 310058, China.
Abstract:
Screening and identifying active compounds from traditional Chinese medicine (TCM) and other natural products plays an important role in drug discovery. Here, we describe a magnetic beads-based multi-target affinity selection-mass spectrometry approach for screening bioactive compounds from natural products. Key steps and parameters including activation of magnetic beads, enzyme/protein immobilization, characterization of functional magnetic beads, screening and identifying active compounds from a complex mixture by LC/MS, are illustrated. The proposed approach is rapid and efficient in screening and identification of bioactive compounds from complex natural products.
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