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A portable automatic endpoint detection system for amplicons of loop mediated isothermal amplification on
Shah Mukim Uddin1,2, Fatimah Ibrahim3,4, Abkar Ahmed Sayad1,2
1Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. thongkl@um.edu.my.
Sensors (Basel, Switzerland)
|March 10, 2015
Summary
A new automated system detects foodborne pathogens using loop-mediated isothermal amplification (LAMP) on a microfluidic disk. This rapid, point-of-care device offers sensitive Salmonella detection, crucial for food safety.
Area of Science:
- Food Science
- Biosensor Technology
- Molecular Diagnostics
Background:
- Foodborne illnesses pose significant public health risks.
- Rapid and accurate detection of foodborne pathogens is essential for food safety.
- Existing detection methods can be time-consuming or require specialized laboratory equipment.
Purpose of the Study:
- To develop an automated endpoint detection system for loop-mediated isothermal amplification (LAMP) amplicons.
- To integrate this system with a microfluidic compact disk platform for pathogen detection.
- To create a rapid, point-of-care diagnostic tool for foodborne pathogens.
Main Methods:
- Utilized a monochromatic ultraviolet (UV) emitter for fluorescent amplicon excitation.
- Employed a color sensor to detect fluorescence emitted from target DNA.
- Developed an automated system to process sensor output and display results on an LCD.
- Performed sensitivity tests using varying DNA concentrations of Salmonella bacteria.
Main Results:
- Achieved a detection limit as low as 2.5 × 10(-3) ng/µL for Salmonella DNA.
- Demonstrated a rapid and automatic endpoint detection capability.
- Successfully integrated LAMP amplification with a microfluidic compact disk platform.
Conclusions:
- The developed system provides a sensitive and rapid method for foodborne pathogen detection.
- This automated detection system has the potential for point-of-care applications.
- The technology is suitable for resource-limited environments, enhancing food safety surveillance.
