Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

868
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
868
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.3K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of nerve fibers in the oviduct isthmus in women with hydrosalpinx.

Acta histochemica·2013
Same author

[Effect of P2X7 receptor on inflammatory diseases and its mechanism].

Sheng li xue bao : [Acta physiologica Sinica]·2013
Same author

Analysis of accuracy in pointing with redundant hand-held tools: a geometric approach to the uncontrolled manifold method.

PLoS computational biology·2013
Same author

In vitro cytotoxicity of CdSe/ZnS quantum dots with different surface coatings to human keratinocytes HaCaT cells.

Journal of environmental sciences (China)·2013
Same author

Diminished appetite predicts mortality of Chinese peritoneal dialysis patients.

Biological research for nursing·2013
Same author

Chiral induction and amplification in supramolecular systems at the liquid-solid interface.

Chemphyschem : a European journal of chemical physics and physical chemistry·2013

Related Experiment Video

Updated: Apr 16, 2026

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
08:55

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

2.7K

[Identification of Dendrobii Caulis basing on ITS sequence].

Zi Ye, Ye Lu, Zheng-Tao Wang

    Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
    |March 11, 2015
    PubMed
    Summary

    DNA identification of Dendrobii Caulis is crucial. A modified CTAB method successfully isolated DNA from dried samples, enabling accurate species identification via ITS sequencing, surpassing traditional methods.

    More Related Videos

    A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
    12:01

    A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

    Published on: February 20, 2012

    32.4K
    Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
    13:16

    Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

    Published on: January 22, 2018

    22.5K

    Related Experiment Videos

    Last Updated: Apr 16, 2026

    Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
    08:55

    Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

    Published on: November 1, 2024

    2.7K
    A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
    12:01

    A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

    Published on: February 20, 2012

    32.4K
    Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
    13:16

    Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

    Published on: January 22, 2018

    22.5K

    Area of Science:

    • Molecular Biology
    • Botany
    • Pharmacognosy

    Context:

    • Accurate identification of Dendrobii Caulis (Dendrobium) is essential for its medicinal use.
    • Traditional identification methods for crude drugs face limitations with processed and dried samples.
    • Genomic DNA isolation is a prerequisite for molecular identification.

    Purpose:

    • To develop and validate a reliable method for isolating high-quality genomic DNA from dried Dendrobii Caulis.
    • To utilize DNA sequencing of the ITS region for precise identification of Dendrobium species.
    • To compare the efficacy of different DNA isolation techniques.

    Summary:

    • A modified CTAB method was optimized for efficient genomic DNA extraction from large samples of dried Dendrobii Caulis (including Huangcao and Fengdou).
    • Internal Transcribed Spacer (ITS) regions were amplified, cloned, and sequenced using the extracted DNA.
    • Sequence comparison against GenBank and in-house data successfully identified multiple Dendrobium species and related taxa from both "Huangcao" and "Fengdou" samples.

    Impact:

    • Establishes a robust DNA-based molecular identification method for dried Dendrobii Caulis.
    • Overcomes the limitations of traditional morphological and organoleptic identification techniques.
    • Demonstrates significant application prospects for ensuring the quality and authenticity of Dendrobium crude drugs.