Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.8K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.8K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.9K
Electrophoresis: Overview01:20

Electrophoresis: Overview

5.2K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
5.2K
Gas Chromatography: Sample Injection Systems01:08

Gas Chromatography: Sample Injection Systems

2.1K
In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
Two primary injection methods are used...
2.1K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

2.1K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The revolver halo as a forensic marker: Raman spectroscopic evidence of primer-driven gunshot residue deposition.

Forensic science international·2026
Same author

Evaluating Coronavirus Stability: Insights From Raman Spectroscopy and Multivariate Analysis.

Journal of biophotonics·2026
Same author

Combination of two HSI cameras and chemometrics for discriminating bodily, non-bodily, and mixed stains.

Forensic science international·2026
Same author

Understanding Liver and Digestive Diseases: A Paved Road to Improve Diagnosis, Management, and Treatment.

Exploration of digestive diseases·2026
Same author

Aging discrimination of multispecies bloodstains on realistic forensic substrates using vibrational spectroscopy and chemometric models.

Forensic science international·2026
Same author

Prenols and prenoic acids in metabolism, disease, and aging.

Journal of lipid research·2026
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

10.6K

Micro-injector for capillary electrophoresis.

Jorge Sáiz1,2,3, Israel Joel Koenka3, Carmen García-Ruiz1,2

  • 1Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá, Alcalá de Henares, Madrid, Spain.

Electrophoresis
|March 11, 2015
PubMed
Summary
This summary is machine-generated.

A new micro-injector for capillary electrophoresis enables precise analysis of small sample volumes (300 nL). This automated system achieves fast separation of inorganic cations with excellent stability and detection limits.

Keywords:
Capillary electrophoresisMicro-injectorMicrofluidicsOral fluid

More Related Videos

Microinjectrode System for Combined Drug Infusion and Electrophysiology
08:30

Microinjectrode System for Combined Drug Infusion and Electrophysiology

Published on: November 13, 2019

7.4K
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
08:04

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Published on: December 18, 2017

15.2K

Related Experiment Videos

Last Updated: Apr 16, 2026

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

10.6K
Microinjectrode System for Combined Drug Infusion and Electrophysiology
08:30

Microinjectrode System for Combined Drug Infusion and Electrophysiology

Published on: November 13, 2019

7.4K
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
08:04

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Published on: December 18, 2017

15.2K

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Limited sample volume is a significant challenge in capillary electrophoresis (CE).
  • Existing injection methods may not be suitable for ultra-low volume samples.

Purpose of the Study:

  • To design and construct a novel micro-injector for CE capable of handling sample volumes as low as 300 nL.
  • To automate sample injection and capillary flushing processes for enhanced efficiency.

Main Methods:

  • A micro-injector system was developed, utilizing controlled compressed air pressurization for sample introduction.
  • Automated flushing of the injection chamber and capillary was integrated.
  • The system was tested with a 25 μm capillary and a contactless conductivity detector.

Main Results:

  • The micro-injector demonstrated stable migration times and peak areas.
  • Fast separation (< 3 min) of five inorganic cations (Na+, K+, NH4+, Ca2+, Mg2+) was achieved.
  • Good limits of detection (10 μM) and linear ranges (10–1000 μM) were obtained.

Conclusions:

  • The novel micro-injector is effective for ultra-low volume CE analyses.
  • The system allows for rapid and sensitive determination of inorganic cations.
  • It was successfully applied to analyze inorganic cations in lake sediment porewater samples.