Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stability of CAT gene transcript depends on the 3'-end structure.

K Sato1, M Yagi, T Nohno

  • 1Department of Biochemistry, Kawasaki Medical School, Kurashiki, Japan.

Nucleic Acids Symposium Series
|January 1, 1989
PubMed
Summary

The 3'-end structure of messenger RNA (mRNA) influences gene expression regulation in eukaryotic cells. Specific mRNA 3'-end structures affect stability, impacting gene regulation, especially after phorbol ester treatment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutron-Multiplicity Measurement in Muon Capture on Oxygen Nuclei in the Gadolinium-Loaded Super-Kamiokande Detector.

Physical review letters·2026
Same author

Results from the T2K Experiment on Neutrino Mixing Including a New Far Detector μ-like Sample.

Physical review letters·2026
Same author

First Differential Measurement of the Single π^{+} Production Cross Section in Neutrino Neutral-Current Scattering.

Physical review letters·2025
Same author

First Measurement of the Electron-Neutrino Charged-Current Pion Production Cross Section on Carbon with the T2K Near Detector.

Physical review letters·2025
Same author

First Joint Oscillation Analysis of Super-Kamiokande Atmospheric and T2K Accelerator Neutrino Data.

Physical review letters·2025
Same author

Search for Periodic Time Variations of the Solar ^{8}B Neutrino Flux between 1996 and 2018 in Super-Kamiokande.

Physical review letters·2024

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Eukaryotic Gene Expression

Background:

  • The 3'-end structure of mRNA plays a crucial role in post-transcriptional gene regulation.
  • Understanding how these structures influence mRNA stability and translation is key to deciphering gene expression control.

Purpose of the Study:

  • To investigate the role of various eukaryotic 3'-end mRNA structures in regulating gene expression.
  • To determine if specific 3'-end elements affect mRNA stability and response to stimuli like phorbol ester.

Main Methods:

  • Construction of pSCAT plasmids containing the chloramphenicol acetyltransferase (CAT) gene fused to different eukaryotic 3'-regulatory elements.
  • Transfection of cells with these plasmids and measurement of CAT activity and mRNA levels.

Related Experiment Videos

  • Treatment of transfected cells with phorbol ester (TPA) to assess mRNA stability.

    • Main Results:

    • CAT activity and mRNA levels were well correlated, indicating the 3'-end structures influenced gene expression.
    • Polyadenylated CAT mRNAs from certain genes were stabilized upon TPA treatment.
    • mRNAs with 3'-end structures from histone or C-myc genes did not show stabilization after TPA treatment.

    Conclusions:

    • The 3'-end structure of mRNA is a significant factor in eukaryotic gene expression regulation.
    • Specific mRNA 3'-end elements differentially affect mRNA stability and cellular responses to external stimuli.