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Related Experiment Video

Updated: Apr 16, 2026

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

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Published on: July 17, 2017

Conditional-ready mouse embryonic stem cell derived macrophages enable the study of essential genes in macrophage

A T Y Yeung¹, C Hale¹, J Xia²

¹Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.

Scientific Reports

|March 11, 2015

Summary

Mouse embryonic stem (ES) cells can differentiate into macrophages for studying host-pathogen interactions. Traf2-deficient macrophages showed altered inflammatory responses and increased susceptibility to S. Typhimurium infection.

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Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

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Published on: June 23, 2023

Area of Science:

Immunology
Stem Cell Biology
Microbiology

Background:

Mouse embryonic stem (ES) cells offer a valuable in vitro model for studying macrophage function and host-pathogen interactions.
This approach circumvents the need for animal experimentation, especially when studying essential genes unavailable in knockout models.

Purpose of the Study:

To differentiate mouse ES cells into functional macrophages and assess their response to S. Typhimurium infection.
To investigate the role of Traf2 in macrophage response to inflammatory stimuli and bacterial infection.

Main Methods:

In vitro differentiation of mouse ES cells into macrophages.
Flow cytometry, microscopic imaging, and RNA-Sequencing (RNA-Seq) for cellular and molecular analysis.
Construction and analysis of a homozygous mutant mouse ES cell line deficient in the Traf2 gene.

Main Results:

ES cell-derived macrophages exhibited comparable responses to S. Typhimurium as bone marrow-derived macrophages.
Traf2-deficient macrophages displayed reduced inflammatory cytokine production upon lipopolysaccharide (LPS) or flagellin stimulation.
Traf2-deficient macrophages showed increased susceptibility to S. Typhimurium infection.

Conclusions:

In vitro differentiated ES cell-derived macrophages serve as a viable model for infection studies.
Traf2 plays a significant role in regulating macrophage inflammatory responses and defense against S. Typhimurium.