Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Mass Spectrometry: Overview

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Food-related allergic reactions in a school setting with a strict management plan.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2025

Same author

The Severity of Allergic Reactions in a Real-World Environment Is Independent of the Eliciting Amounts of Foods.

Allergy·2024

Same author

Clinical method evaluation of hemoglobin S and C identification by top-down selected reaction monitoring and electron transfer dissociation.

Clinical proteomics·2020

Same author

Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions.

Methods in molecular biology (Clifton, N.J.)·2019

Same author

Detection of busulfan adducts on proteins.

Rapid communications in mass spectrometry : RCM·2016

Same author

Multicenter experiment for quality control of peptide-centric LC-MS/MS analysis - A longitudinal performance assessment with nLC coupled to orbitrap MS analyzers.

Journal of proteomics·2015

Search research articles

Related Experiment Video

Updated: Apr 16, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

Clinical protein mass spectrometry.

Alexander Scherl¹

¹Department of Human Protein Science, Faculty of Medicine, University of Geneva, Geneva, Switzerland; Department of Genetic and Laboratory Medicine, Geneva University Hospitals, Geneva, Switzerland.

Methods (San Diego, Calif.)

|March 11, 2015

Summary

This summary is machine-generated.

Mass spectrometry (MS) offers high specificity for protein analysis in clinical labs, surpassing traditional immunoassays. While MS is a developing technology, its unique capabilities promise future advancements in diagnostic protein quantification.

Keywords:

Clinical diagnostics Mass spectrometry Proteins Top-down proteomics

More Related Videos

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Related Experiment Videos

Last Updated: Apr 16, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Area of Science:

Clinical Chemistry
Analytical Chemistry
Biotechnology

Background:

Quantitative protein analysis is crucial for clinical diagnosis, monitoring, and prognosis.
Current protein assays often rely on non-specific methods or immunoassays.
Mass spectrometry (MS) presents a highly specific alternative for protein quantification.

Purpose of the Study:

To provide a didactic overview of mass spectrometry (MS) assays in clinical laboratories.
To compare the advantages and disadvantages of MS assays versus immunoassays.
To explore current and potential future applications of protein MS in clinical settings.

Main Methods:

Review of mass spectrometry instrumentation and methodologies for protein analysis.
Comparison of top-down (intact protein) and bottom-up (peptide) MS approaches.
Analysis of existing and proposed MS-based protein assays for clinical use.

Main Results:

Mass spectrometry offers superior specificity, detecting protein variants, modifications, and degradation.
Protein MS is a relatively new field requiring specialized expertise and instrumentation.
Currently, few protein MS applications are routinely implemented in clinical chemistry labs.

Conclusions:

Mass spectrometry holds significant potential to enhance the specificity of clinical protein analysis.
Further development and adoption of MS technologies are needed for widespread clinical use.
MS offers distinct advantages over immunoassays for specific protein analyses in the future.