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Ultrastructural changes in rat liver sinusoids during storage in cold Euro-Collins solution
1Department of Surgery I, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Virchows Archiv. B, Cell Pathology Including Molecular Pathology
|January 1, 1989
Summary
Cold storage of rat livers damages sinusoidal endothelial cells, leading to blebs and microcirculation issues. Prolonged preservation impacts liver viability for transplantation.
Area of Science:
- Hepatology
- Transplantation Biology
- Microcirculation Research
Background:
- Organ preservation is critical for transplantation success.
- Understanding ultrastructural changes during cold ischemia is essential for improving graft survival.
Purpose of the Study:
- To investigate the ultrastructural changes in rat liver sinusoids during cold storage.
- To assess the impact of preservation time on sinusoidal endothelial cells and hepatocytes.
Main Methods:
- Rat livers were stored in Euro-Collins solution at 2°C for varying durations.
- Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used for ultrastructural analysis.
Main Results:
- Cold ischemia damaged sinusoidal endothelial cells, with fenestrations enlarging and sieve plates being destroyed after 8 hours.
- Hepatocyte blebs increased with preservation time, filling sinusoids after 24 hours.
- Uneven fixation and circulatory disturbances were noted after 12 hours, but parenchymal cells showed no irreversible damage.
Conclusions:
- Endothelial cell destruction and hepatocyte blebs negatively affect microcirculation in preserved livers.
- Prolonged cold storage can compromise the viability of transplanted livers due to sinusoidal damage.