Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microtubules in Signaling01:22

Microtubules in Signaling

2.3K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
2.3K
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

7.6K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
7.6K
Centrosome Duplication02:25

Centrosome Duplication

5.2K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
5.2K
Centrosome Duplication02:25

Centrosome Duplication

3.0K
3.0K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.8K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.8K
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

5.9K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysine demethylase 4A: from chromatin to centrosomes.

The FEBS journal·2025
Same author

Drug and siRNA screens identify ROCK2 as a therapeutic target for ciliopathies.

Communications medicine·2025
Same author

Author Correction: Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner.

Nature communications·2024
Same author

Preclinical Development of T Cells Engineered to Express a T-Cell Antigen Coupler Targeting Claudin 18.2-Positive Solid Tumors.

Cancer immunology research·2024
Same author

Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner.

Nature communications·2024
Same author

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis.

eLife·2023

Related Experiment Video

Updated: Apr 16, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.9K

Centrin2 regulates CP110 removal in primary cilium formation.

Suzanna L Prosser1, Ciaran G Morrison2

  • 1Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland, Galway, Galway, Ireland.

The Journal of Cell Biology
|March 11, 2015
PubMed
Summary

Centrin2 is essential for primary cilia formation in B lymphocytes and other cells. This protein regulates ciliogenesis by controlling levels of the ciliation inhibitor CP110.

More Related Videos

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
14:19

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Published on: March 25, 2022

4.5K
Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
07:07

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Published on: February 21, 2016

11.1K

Related Experiment Videos

Last Updated: Apr 16, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.9K
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
14:19

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Published on: March 25, 2022

4.5K
Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
07:07

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Published on: February 21, 2016

11.1K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Primary cilia are crucial sensory microtubule structures involved in cellular signaling.
  • The precise regulation of basal body formation during ciliogenesis remains incompletely understood.
  • Centrin2, a calcium-binding protein, has been investigated for its potential role in ciliogenesis.

Purpose of the Study:

  • To investigate the role of centrin2 in the process of ciliogenesis.
  • To determine if centrin2 is required for primary cilia formation in lymphocytes.
  • To elucidate the molecular mechanism by which centrin2 regulates ciliogenesis.

Main Methods:

  • Reverse genetics was employed to study centrin2 function.
  • Chicken DT40 B lymphocytes and human retinal pigmented epithelial cells were used as model systems.
  • Serum starvation was utilized to induce cellular quiescence and promote ciliogenesis.
  • Centrin2 gene disruption (CETN2) and CP110 knockdown were performed.

Main Results:

  • Efficient primary ciliogenesis in chicken DT40 B lymphocytes was dependent on centrin2.
  • Disruption of CETN2 in human cells prevented cilia formation despite intact centrioles.
  • CETN2-deficient cells exhibited abnormal distal appendage protein localization and failed to downregulate CP110.
  • CP110 knockdown rescued ciliogenesis in CETN2-deficient cells.

Conclusions:

  • Centrin2 is a critical regulator of primary ciliogenesis.
  • Centrin2 controls primary cilia formation by modulating the levels of the ciliation inhibitor CP110.
  • These findings reveal a novel mechanism for regulating ciliogenesis involving centrin2 and CP110.