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Fast and sensitive detection of indels induced by precise gene targeting

Zhang Yang1, Catharina Steentoft2, Camilla Hauge3

  • 1Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark Novo Nordisk Foundation Center for Biosustainability, Danish Technical University, Lyngby, Denmark.

Nucleic Acids Research
|March 11, 2015
PubMed
Summary

We developed Indel Detection by Amplicon Analysis (IDAA), a fast and sensitive method for detecting small DNA insertions and deletions (indels) after gene editing. This technique accurately measures indel sizes for high-throughput genomic analysis.

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