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Updated: Apr 16, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Synergism of regulatory elements in σ(B)- and σ(A)-dependent promoters enhances recombinant protein expression in
Teva Phanaksri1, Plearnpis Luxananil2, Sakol Panyim3
1Institute of Molecular Biosciences, Mahidol University, Phutthamontol, Nakhon Pathom 73170, Thailand.
Abstract:
Strong promoter is an essential factor for production of recombinant protein in various expression systems including Bacillus subtilis. In this study, we described a strategy to improve the expression efficiency using synthetic double promoter. Assembly of the conserved elements from σ(B)- and σ(A)-dependent promoters constitutively improved the yield of recombinant protein approximately 2-3-fold in both exponential and stationary growth phase. The synergistic effect in the double promoter was observed only when σ(B)-promoter was located upstream to σ(A)-promoter but independent to its orientation. A conserved element in either -10 or -35 box of σ(B)-promoter is sufficient to promote the synergism. Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.
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