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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Persistent aggregates in apheresis platelet concentrates.

H B Feys1, J Coene, R Devloo

  • 1Transfusion Research Center, Belgian Red Cross-Flanders, Ghent, Belgium.

Vox Sanguinis
|March 11, 2015
PubMed
Summary
This summary is machine-generated.

Persistent aggregates (PA) in apheresis concentrates are linked to specific donors with higher platelet counts. While product quality remains acceptable, further functional studies are recommended.

Keywords:
apheresisblood collectionplatelet concentrates

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Area of Science:

  • Hematology
  • Transfusion Medicine
  • Biotechnology

Background:

  • Apheresis procedures can generate persistent aggregates (PA) in platelet concentrates.
  • These aggregates may persist during storage, leading to product wastage.
  • Understanding the factors contributing to PA formation is crucial for improving platelet product quality.

Purpose of the Study:

  • To assess the product quality of apheresis concentrates containing persistent aggregates (PA).
  • To identify donor-related factors associated with the formation of PA.

Main Methods:

  • Comparison of platelet indices and donation parameters between apheresis concentrates with and without PA (n=180).
  • Analysis of donor whole blood platelet counts, apheresis yields, and product quality parameters (pH, lactic acid, cytokines).
  • Flow cytometry assessment of GPIbα levels, phosphatidylserine exposure, integrin activation, and degranulation (P-selectin expression).
  • Functional studies including aggregation assays and agglutination tests.

Main Results:

  • Donor dependence for PA formation was observed, with a higher proportion of donors having previous PA donations in the PA group.
  • Donors in the PA group had significantly higher whole blood platelet counts and apheresis yields.
  • PA products showed lower pH and higher lactic acid concentrations post-donation.
  • Increased integrin activation and degranulation (P-selectin) were noted in PA products, along with higher cytokine concentrations.
  • Agglutination at low-dose ristocetin was significantly higher in PA products, while aggregation responses to peptide and collagen were normal.

Conclusions:

  • Apheresis concentrates with PA exhibit acceptable quality parameters but require further functional investigation.
  • PA formation is more likely to recur in donors with elevated platelet counts.
  • Identifying and managing donors prone to PA formation can help reduce product wastage.