Related Experiment Video

Updated: Apr 16, 2026

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
09:23

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

2.8K

[Genetic and molecular principles for the selection of Pseudomonas and Staphylococcus therapeutic bacteriophages]

K A Miroshnikov, E E Kulikov, O S Darbeeva

    Prikladnaia Biokhimiia I Mikrobiologiia
    |March 12, 2015
    PubMed

    Insights

    This study investigated bacteriophage mixtures for treating Staphylococcus and Pseudomonas infections. Genotyping is proposed for accurate identification of therapeutic bacteriophages.

    Area of Science:

    • Microbiology
    • Virology
    • Bioinformatics

    Context:

    • Bacteriophage therapy is a promising alternative to antibiotics for bacterial infections.
    • Phage mixtures are used for treating staphylococcal and pseudomonade infections.
    • Accurate identification of therapeutic phages is crucial for efficacy and safety.

    Purpose:

    • To investigate the composition of empirically selected bacteriophage mixtures.
    • To assess the suitability of phages for therapeutic use based on genomic and morphological analysis.
    • To propose a method for precise phage identification.

    Summary:

    • Negative stain electron microscopy and bioinformatics analysis were used to characterize bacteriophage mixtures.
    • Most phages belonged to groups suitable for therapeutic applications against Staphylococcus and Pseudomonas.
    • Morphological analysis alone was insufficient for exact phage group identification, highlighting the need for genetic methods.

    Impact:

    • Suggests group genotyping based on conserved genetic loci for accurate characterization of therapeutic bacteriophages.
    • Aims to improve the quality control and reliability of phage-based treatments.
    • Contributes to the advancement of phage therapy as a viable medical intervention.

    More Related Videos

    Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings07:22

    Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

    Published on: August 19, 2021

    3.6K
    Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos11:20

    Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos

    Published on: May 12, 2020

    7.1K

    Related Concept Videos

    Lytic Cycle of Bacteriophages01:30

    Lytic Cycle of Bacteriophages

    Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
    80.7K
    Antibiotic Selection00:57

    Antibiotic Selection

    Overview
    62.3K
    DNA Bacteriophages01:26

    DNA Bacteriophages

    Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
    1.6K
    Clinical Significance of Antibiotic Resistance01:25

    Clinical Significance of Antibiotic Resistance

    Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
    60
    Gene Regulation in Microbial Communities: Quorum Sensing01:28

    Gene Regulation in Microbial Communities: Quorum Sensing

    Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
    924
    Microorganisms in Medicine and Therapeutics01:29

    Microorganisms in Medicine and Therapeutics

    Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
    1.5K