Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1.5K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.5K
Gene Therapy00:59

Gene Therapy

28.2K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
28.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted in vivo delivery of genetic medicines utilizing an engineered lentiviral vector platform results in CAR T and NK cell generation.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Protection against SIV in Rhesus Macaques Using Albumin and CD4-Based Vector-Mediated Gene Transfer.

Molecular therapy. Methods & clinical development·2020
Same author

Adeno-associated virus vectored immunoprophylaxis to prevent HIV in healthy adults: a phase 1 randomised controlled trial.

The lancet. HIV·2019
Same author

Engineered Expression of Broadly Neutralizing Antibodies Against Human Immunodeficiency Virus.

Annual review of virology·2017
Same author

Recombinant Adeno-Associated Virus Vector Genomes Take the Form of Long-Lived, Transcriptionally Competent Episomes in Human Muscle.

Human gene therapy·2015
Same author

Emerging Vaccine Technologies.

Vaccines·2015

Related Experiment Video

Updated: Apr 16, 2026

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

10.4K

Vector-mediated antibody gene transfer for infectious diseases.

Bruce C Schnepp1, Philip R Johnson

  • 1Infectious Disease, The Children's Hospital of Philadelphia, Abramson Research Center, Room 1216J, 3615 Civic Center Blvd., Philadelphia, PA, 19104, USA, schnepp@email.chop.edu.

Advances in Experimental Medicine and Biology
|March 12, 2015
PubMed
Summary

Vector-mediated antibody gene transfer offers a novel vaccine strategy for infectious diseases like HIV. This method uses recombinant adeno-associated virus vectors for long-term antibody expression, improving upon passive immunization.

More Related Videos

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

14.5K
Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

5.6K

Related Experiment Videos

Last Updated: Apr 16, 2026

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

10.4K
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

14.5K
Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

5.6K

Area of Science:

  • Immunology and Virology
  • Gene Therapy and Vaccine Development

Background:

  • Classical passive immunization has limitations in providing long-term protection against infectious diseases.
  • Developing effective vaccines for challenging pathogens like HIV, Hepatitis C, malaria, RSV, and tuberculosis remains a significant hurdle.

Purpose of the Study:

  • To explore vector-mediated antibody gene transfer as a novel vaccine approach for infectious diseases.
  • To highlight the potential of this strategy for HIV-1 and other difficult-to-vaccinate targets.

Main Methods:

  • Utilizing recombinant adeno-associated virus (rAAV) vectors to deliver antibody genes directly to the host.
  • Achieving long-term, endogenous antibody expression from injected muscle tissue.

Main Results:

  • Demonstrated successful long-term antibody expression conferring protective immunity.
  • Gene transfer products showed potency and breadth identical to original antibodies.
  • This approach bypasses the need for immunogen design and adaptive immune system interaction.

Conclusions:

  • Vector-mediated antibody gene transfer represents a promising alternative to traditional vaccines and passive immunization.
  • This methodology can be rapidly translated to clinical applications, particularly for HIV-1.
  • The strategy offers a new paradigm for generating protective immunity against various infectious agents.