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A two-photon fluorescent probe for detecting endogenous hypochlorite in living cells
Xunming Wang1, Xiaohui Wang, Yan Feng
1Department of Chemistry, Anhui University, Hefei, 230601, China. mengxm@ahu.edu.cn.
Dalton Transactions (Cambridge, England : 2003)
|March 12, 2015
Summary
A new fluorescent probe detects hypochlorite (ClO(-)) with enhanced sensitivity and selectivity. This tool aids in discovering drugs that modulate microglia activity for potential therapeutic applications.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Chemical Biology
Background:
- Hypochlorite (ClO(-)) is a reactive oxygen species involved in various biological processes.
- Accurate detection of hypochlorite is crucial for understanding its role in cellular functions and diseases.
- Existing detection methods may lack sensitivity, selectivity, or biocompatibility for in vivo applications.
Purpose of the Study:
- To develop a novel two-photon fluorescent probe for sensitive and selective detection of hypochlorite.
- To evaluate the probe's performance in detecting intracellular hypochlorite in live cells.
- To demonstrate the probe's utility in identifying potential therapeutic agents targeting microglia.
Main Methods:
- Synthesis of a novel two-photon fluorescent probe based on ClO(-)-promoted oxidation of a C=N bond.
- Characterization of the probe's photophysical properties and fluorescence response to ClO(-).
- In vitro and in vivo experiments using BV-2 cells stimulated with lipopolysaccharide (LPS) for intracellular hypochlorite detection.
Main Results:
- The developed probe exhibited a linear fluorescence response to hypochlorite with a 23.5-fold enhancement.
- High selectivity and sensitivity were achieved for two-photon fluorescence detection of hypochlorite.
- The probe demonstrated good biocompatibility, enabling intracellular hypochlorite monitoring in live BV-2 cells.
- Successful application in monitoring endogenous hypochlorite in LPS-stimulated microglia.
Conclusions:
- A novel, highly sensitive, and selective two-photon fluorescent probe for hypochlorite detection has been successfully developed.
- The probe is suitable for real-time monitoring of intracellular hypochlorite in biological systems.
- This probe serves as a valuable tool for high-throughput screening of pharmacological microglia modulators, advancing research in neuroinflammation and related diseases.
