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microRNA-107 functions as a candidate tumor suppressor gene in renal clear cell carcinoma involving multiple genes

Nan Song1, Xin Ma1, Hongzhao Li1

  • 1Department of Urology, Chinese PLA General Hospital, Beijing, China.

Urologic Oncology
|March 12, 2015
PubMed
Abstract

Insights

MicroRNA-107 (miR-107) suppresses clear cell renal cell carcinoma by inhibiting cell proliferation and invasion. This finding offers a potential therapeutic strategy for renal cell carcinoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
  • miRNAs play dual roles as oncogenes or tumor suppressors in cancer.
  • Dysregulation of miRNAs is implicated in tumorigenesis and cancer progression.

Purpose of the Study:

  • To investigate the role of microRNA-107 (miR-107) in clear cell renal cell carcinoma (ccRCC).
  • To explore the potential of miR-107 as a therapeutic agent for ccRCC.

Main Methods:

  • Analysis of miR-107 expression in 52 paired clinical ccRCC specimens and cell lines.
  • Overexpression of miR-107 using lentiviral vectors and biosynthesis mimics in vitro and in vivo.
  • Assessment of cell proliferation, invasion, cell cycle, and tumor growth.
  • Identification of direct miR-107 targets using molecular assays.

Main Results:

  • miR-107 exhibited tumor-suppressing functions in ccRCC.
  • miR-107 overexpression inhibited proliferation and invasiveness of ccRCC cells (786-O).
  • High miR-107 levels induced G2/M phase cell cycle arrest and retarded tumor growth in vivo.
  • Eukaryotic translation initiation factor 5 was identified as a direct target of miR-107.
  • miR-107 expression was associated with p53 and VHL genes in ccRCC.

Conclusions:

  • miR-107 acts as a tumor suppressor in ccRCC by inhibiting cell proliferation and invasiveness.
  • miR-107 holds potential as a therapeutic target for ccRCC treatment.
Keywords:
EIF5MicroRNARenal cell carcinomaVHLmiR-107p53

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