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In vitro development of metacyclic Trypanosoma simiae derived from bloodstream trypomastigotes
E Zweygarth1, I D Gumm, M A Gray
1Kenya Trypanosomiasis Research Institute, Kikuyu, Kenya.
Acta Tropica
|October 1, 1989
Abstract:
A stock of Trypanosoma simiae was transformed into procyclic forms at 26 C in a semi-defined maintenance medium. After transformation, the trypanosomes were maintained in a modified Eagle's MEM medium. On day 35 of cultivation, epimastigotes attached to the bottom of the culture flask. From day 44 onwards, metacyclic-like trypanosomes were observed. Subcutaneous injections into pigs of trypanosome suspensions obtained from cultures on day 10 were not infective, whereas culture-derived metacyclics (days 44, 63 and 69) were highly pathogenic.