Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.2K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.2K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Species, cell proliferation state and oxygen partial pressure are major factors in the in vitro toxicity of repurposed potential ADPKD drugs in human and murine renal proximal tubule cells.

Archives of toxicology·2026
Same author

Changes in HDAC Expression and Activity by Oolongtheanin Digallate and Theasinensins and the Onset of Apoptosis.

Molecules (Basel, Switzerland)·2026
Same author

Chemical, Biological, and Ecological Evidence for Aerobic Deoxynivalenol Detoxification in Agronomic Soil-Derived Bacterial Communities.

Toxins·2026
Same author

The activation of the metabolic oxaloacetate-pyruvate axis restores influenza A virus replication during impaired glycolysis.

Virology journal·2026
Same author

Planting date impacts disease progression and yield of chickpea (<i>Cicer arietinum</i> L.) grown in the Northern Great Plains.

Plant disease·2026
Same author

Matrix Effect Variability in Urine Samples from Different Cohorts and Implications on LC-ESI-MS Mycotoxin Biomarker Analysis.

Toxins·2026

Related Experiment Video

Updated: Apr 16, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

18.9K

A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples.

Sebastian Hickert1, Johannes Gerding, Edson Ncube

  • 1Institute of Food Chemistry, University of Münster, Corrensstraße 45, 48149, Münster, Germany.

Mycotoxin Research
|March 12, 2015
PubMed
Summary

A new, cost-effective method using HPLC-MS/MS can quantify 26 mycotoxins in grain products. This rapid, simple technique offers high recovery rates for regulated and other mycotoxins, aiding food safety analysis.

More Related Videos

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.7K
Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
07:10

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

11.0K

Related Experiment Videos

Last Updated: Apr 16, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
10:01

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

Published on: April 23, 2012

18.9K
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.7K
Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
07:10

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

11.0K

Area of Science:

  • Analytical Chemistry
  • Food Science
  • Toxicology

Background:

  • Mycotoxins pose significant risks to food safety and human health.
  • Accurate and efficient detection methods are crucial for monitoring mycotoxin contamination in food products.

Purpose of the Study:

  • To develop a simple, fast, and cost-effective method for the quantitative determination of 26 mycotoxins using HPLC-MS/MS.
  • To validate the method's performance for regulated and non-regulated mycotoxins in grain products.

Main Methods:

  • Development of a micro high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method.
  • Utilized a single extraction step with a dilute-and-shoot approach, eliminating the need for further cleanup.
  • Achieved a total run time of 9 minutes with minimal solvent consumption (<0.3 mL per run).

Main Results:

  • Successfully quantified 26 mycotoxins, including those regulated by the European Commission (e.g., fumonisins, aflatoxins, deoxynivalenol, ochratoxin A, T-2/HT-2 toxins, zearalenone) and others (e.g., enniatins, beauvericin, Alternaria toxins).
  • Demonstrated high recoveries for regulated compounds (76-120%) and acceptable recoveries for other toxins (≥51%).
  • Applied the method to analyze 42 maize samples from South African field trials.

Conclusions:

  • The developed HPLC-MS/MS method is a rapid, cost-effective, and efficient tool for the simultaneous quantification of a broad spectrum of mycotoxins.
  • The method's simplicity and high throughput make it suitable for routine food safety analysis and monitoring programs.
  • This approach aids in ensuring compliance with regulatory limits and protecting public health from mycotoxin exposure.