Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

3.8K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.8K
The Extracellular Matrix01:29

The Extracellular Matrix

13.8K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
13.8K
The Extracellular Matrix01:42

The Extracellular Matrix

91.4K
Overview
91.4K
Collagens are the Major Structural Proteins of ECM01:13

Collagens are the Major Structural Proteins of ECM

6.5K
Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. Collagen fiber is made from fibrous protein subunits linked together to form a long, straight fiber. Collagen fibers, while flexible, have great tensile strength, resist stretching, and give ligaments and tendons their characteristic resilience and strength. These fibers hold connective tissues together, even during the body's movement.
Connective tissue proper includes loose...
6.5K
Extracellular Matrix01:26

Extracellular Matrix

6.8K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
6.8K
Type IV Collagen of Basal Lamina01:05

Type IV Collagen of Basal Lamina

3.4K
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen  forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stromal peroxidasin drives early tumor growth in breast cancer.

iScience·2026
Same author

Therapeutic inhibition of myeloperoxidase with AZD5904 attenuates disease progression in mouse models of early-stage and relapsed multiple myeloma.

Haematologica·2026
Same author

Mer receptor expression promotes multiple myeloma disease development via a cell-extrinsic mechanism.

Experimental hematology·2025
Same author

Age-related mesenchymal stromal cell senescence is associated with progression from MGUS to multiple myeloma.

Leukemia·2025
Same author

Substitutions in Fe<sub>2</sub>P Alloys for Permanent Magnet Applications.

Materials (Basel, Switzerland)·2025
Same author

Review of Indications for Endotracheal Intubation in Burn Patients with Suspected Inhalational Injury.

European burn journal·2024

Related Experiment Video

Updated: Apr 16, 2026

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

11.5K

Peroxidase enzymes regulate collagen extracellular matrix biosynthesis.

Mark O DeNichilo1, Vasilios Panagopoulos1, Timothy E Rayner2

  • 1Breast Cancer Research Unit, Discipline of Surgery, The University of Adelaide, Adelaide, South Australia, Australia.

The American Journal of Pathology
|March 12, 2015
PubMed
Summary

Peroxidase enzymes, like myeloperoxidase and eosinophil peroxidase, stimulate fibroblast migration and collagen production, crucial for tissue repair and fibrosis. Their catalytic activity is essential for extracellular matrix generation.

More Related Videos

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
07:03

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Published on: January 31, 2014

12.4K
Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
08:19

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Published on: April 22, 2019

7.6K

Related Experiment Videos

Last Updated: Apr 16, 2026

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

11.5K
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
07:03

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Published on: January 31, 2014

12.4K
Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
08:19

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Published on: April 22, 2019

7.6K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pathology

Background:

  • Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are heme enzymes associated with fibrotic tissue and cancer.
  • Their direct role in fibroblast recruitment and extracellular matrix (ECM) biosynthesis was previously unclear.

Purpose of the Study:

  • To investigate the profibrogenic capacity of peroxidase enzymes.
  • To elucidate the mechanisms by which peroxidases influence fibroblast behavior and ECM production.

Main Methods:

  • In vitro and in vivo studies using cultured fibroblasts.
  • Analysis of fibroblast binding and internalization of MPO and EPO.
  • Assessment of collagen biosynthesis stimulated by peroxidases.
  • Investigation of the role of prolyl 4-hydroxylase and ascorbic acid.
  • Use of the MPO inhibitor 4-amino-benzoic acid hydrazide.

Main Results:

  • Peroxidase enzymes stimulate fibroblast migration and promote ECM generation.
  • Fibroblasts bind and internalize MPO and EPO.
  • Peroxidases enhance collagen biosynthesis post-translationally via prolyl 4-hydroxylase, independent of ascorbic acid.
  • Inhibition of peroxidase activity blocks collagen biosynthesis.

Conclusions:

  • Peroxidase enzymes possess a conserved profibrogenic capacity.
  • MPO and EPO play a fundamental role in fibroblast recruitment and collagen ECM biosynthesis.
  • These findings have significant implications for fibrotic diseases and tissue repair.