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Related Experiment Video

Updated: Apr 16, 2026

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter
08:29

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Single-cell printing based on impedance detection.

J Schoendube, D Wright1, R Zengerle

  • 1Zurich Instruments AG , Technoparkstrasse 1, 8005 Zurich, Switzerland.

Biomicrofluidics
|March 12, 2015
PubMed
Summary

This study introduces a novel device for label-free single-cell isolation using inkjet printing and impedance flow cytometry. The technology enables precise droplet encapsulation of single cells for advanced single-cell analysis applications.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity.
  • Current methods for single-cell isolation often require labeling or can be complex.
  • There is a need for efficient, label-free methods to isolate single cells.

Purpose of the Study:

  • To develop and demonstrate a novel device for label-free isolation of single living cells.
  • To encapsulate single cells within precisely controlled picoliter droplets.
  • To enable downstream single-cell analysis applications.

Main Methods:

  • Integration of inkjet printing technology with impedance flow cytometry.
  • Real-time detection and velocity measurement of single cells using microfabricated electrodes.
  • Controlled droplet generation with volumes ranging from 500-800 picoliters.
  • Assessment of cell viability post-encapsulation.

Main Results:

  • The device successfully encapsulates single living cells in free-flying picoliter droplets.
  • Droplet volume control was achieved via piezo actuator displacement.
  • High precision in cell detection was demonstrated with low signal variation for uniform beads.
  • Single bead efficiency reached 73% ± 11% at a throughput of approximately 9 events/min.
  • Viability of printed HeLa cells and human primary fibroblasts was maintained for at least eight days.

Conclusions:

  • The developed device offers a promising label-free approach for single-cell isolation.
  • The technology facilitates the encapsulation of viable single cells in controllable picoliter droplets.
  • This method supports the advancement of single-cell analysis by providing a robust and efficient isolation technique.